bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering
Authors
abstract
extracelllar matrix (ecm) materials from mammalian tissues have been broadly used for tissue engineering for animal models. while decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. in this study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construct for cartilage substitution. for this purpose, different decellularization protocols including freeze/thaw cycles followed by treatment with various concentrations of sodium dodecyl sulfate (sds) were used. decellularization was analyzed by histological examination including hematoxylin and eosin and picrosirius staining. treatment with 2%sds for 5 to 8h followed by 4% sds for3h hada significant effect on decellularization process with the minimal effect on the collagen contents. on the other hand, treatment with 1% sds had no effect on cell removal and use of 8% sds for 5 to 8 h resulted in complete elimination of cells and significant decrease in cartilage matrix and collagen contents. this study provides a technique to produce acellular ecm derived from articular bovine cartilage whichmay serve as a xenogenicscaffold for cartilage tissue engineering.
similar resources
Mesenchymal stem cells can survive on the extracellular matrix-derived decellularized bovine articular cartilage scaffold
Objective (s): The scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. The objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...
full textDecellularized Cartilage as a Bioactive Biomaterial for Cartilage Tissue Engineering
Cartilage matrix is a particularly promising acellular material for cartilage regeneration given the evidence supporting its chondroinductive character. The ‘raw materials’ of cartilage matrix can serve as building blocks and signals for enhanced tissue regeneration. These matrices can be created by chemical or physical methods: physical methods disrupt cellular membranes and nuclei but may not...
full textmesenchymal stem cells can survive on the extracellular matrix-derived decellularized bovine articular cartilage scaffold
objective (s): the scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. the objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...
full textMesenchymal stem cells can survive on the extracellular matrix-derived decellularized bovine articular cartilage scaffold.
OBJECTIVE S The scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. The objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (BM-...
full textDevelopment and characterization of decellularized human nasoseptal cartilage matrix for use in tissue engineering.
OBJECTIVES/HYPOTHESIS Reconstruction of cartilage defects in the head and neck can require harvesting of autologous cartilage grafts, which can be associated with donor site morbidity. To overcome this limitation, tissue-engineering approaches may be used to generate cartilage grafts. The objective of this study was to decellularize and characterize human nasoseptal cartilage with the aim of ge...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of veterinary science and technologyجلد ۴، شماره ۱، صفحات ۱-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023